Brand Cheap Sale Venue Caspase-9 Assay Kit Fluorometric with Substrate 1 Ac-LEHD-AFC - /conutrition720889.html,-,$329,Kit,,1,,Industrial Scientific , Lab Scientific Products , Life Science Supplies,Substrate,Fluorometric,with,Ac-LEHD-AFC,Assay,Caspase-9 $329 Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC Substrate - 1 Industrial Scientific Lab Scientific Products Life Science Supplies $329 Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC Substrate - 1 Industrial Scientific Lab Scientific Products Life Science Supplies Brand Cheap Sale Venue Caspase-9 Assay Kit Fluorometric with Substrate 1 Ac-LEHD-AFC - /conutrition720889.html,-,$329,Kit,,1,,Industrial Scientific , Lab Scientific Products , Life Science Supplies,Substrate,Fluorometric,with,Ac-LEHD-AFC,Assay,Caspase-9

Brand Cheap Sale Venue Caspase-9 Assay Kit Fluorometric with Virginia Beach Mall Substrate 1 Ac-LEHD-AFC -

Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC Substrate - 1


Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC Substrate - 1


Product description

Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC substrate General description Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorescence microtiter plate reader. Detection Method: Fluorescence (400 nm excitation filter and 505 nm emission filter) Species Reactivity: Mammalian Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis Storage temp.: -20°C Features amp; Benefits: Simple one-step procedure that takes ~1-2 hours Fast and convenient Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity Kit Components: Cell Lysis Buffer 2X Reaction Buffer LEHD-AFC (1 mM) DTT (1 M)

Caspase-9 Assay Kit, Fluorometric with Ac-LEHD-AFC Substrate - 1

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